Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Caspase 11 (also known as CASP11) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CASP11 antibody. CASP11 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CASP11 Antibody is added and binds to CASP11 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CASP11 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CASP11. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm